1362 Dermal papilla cell-derived extracellular vesicles as the future tools for hair regeneration

Exosome, a micro-vesicle, has role to maintain cell-cell communication in paracrine manner and can induce cell proliferation differentiation. Recently exosome mediated differentiation gain attraction development biology due transfer mRNA miRNA recipient cell. But it remains unclear whether adipose derived stem cells (ASCs) could adopt the dermal papilla (DP) like properties with (DPC-Exo). To understand effect of DPCs-Exo, DPCs-Exo was characterized ASCs monolayeror spheroid cell- cultures were incubated DPC-Exo combination medium specialized for DPCs, CAP1/2FP. Dermal (DPCs) initially evaluated by comparing several genes protein those DPCs via real-time PCR analysis immunostaining, respectively. We also presence hair growth related miRNAs, especially mir-214-5P, mir-218-5p mir-195-5P. Here, we found that miRNAs expression patterns varied from Passage 4 (P4) or P5. Also, CAP1/2FP increase at low concentration propagated inductive gene Versican (vcan), Alpha-smooth muscle actin, (α-sma), Osteopontin (opn) N-Cam (ncam).Immunocytochemical suggest partially upregulated VCAN, α-SMA along maintenance β-catenin which are similar their pattern microRNA, mir-218-5P mir-195-5p ASCs. concluded early stage potential equip ability through mir-RNAs.